Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae).
Am J Bot
; 99(10): e379-90, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-23028003
ABSTRACT
PREMISE OF THE STUDY Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. ⢠METHODS AND RESULTS:
Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. â¢CONCLUSIONS:
These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea.
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1
Banco de datos:
MEDLINE
Asunto principal:
Repeticiones de Microsatélite
/
Etiquetas de Secuencia Expresada
/
Lathyrus
Idioma:
En
Año:
2012
Tipo del documento:
Article