A protein kinase, calcineurin B-like protein-interacting protein Kinase9, interacts with calcium sensor calcineurin B-like Protein3 and regulates potassium homeostasis under low-potassium stress in Arabidopsis.
Plant Physiol
; 161(1): 266-77, 2013 Jan.
Article
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| MEDLINE
| ID: mdl-23109687
ABSTRACT
Potassium (Kâº) is an essential macronutrient for plant growth and development. Previous studies have demonstrated that Calcineurin B-Like Protein1 (CBL1) or CBL9 and CBL-Interacting Protein Kinase23 (CIPK23) regulate K⺠uptake in Arabidopsis (Arabidopsis thaliana) roots by modulating K⺠channel Arabidopsis K⺠Transporter1. In this study, we show that the protein kinase CIPK9 interacts with the calcium sensor CBL3 and plays crucial roles in K⺠homeostasis under low-K⺠stress in Arabidopsis. Arabidopsis wild-type plants showed leaf chlorotic symptoms when grown for 10 d on low-K⺠(100 µM) medium. Here, we show that plants lacking CIPK9 displayed a tolerant phenotype to low-K⺠stress, which still maintained green leaves when the wild-type plants showed typical Kâº-deficient symptoms. Overexpressing lines of CIPK9 resulted in a low-Kâº-sensitive phenotype compared with wild-type plants. Furthermore, CBL2 and CBL3 were identified as upstream regulators of CIPK9. Both CBL2- and CBL3-overexpressing lines displayed similar low-Kâº-sensitive phenotypes and K⺠contents to CIPK9-overexpressing lines. However, only cbl3 mutant plants, but not cbl2 mutant plants, showed the low-Kâº-tolerant phenotype similar to cipk9 mutants. Taken together, these results demonstrate that CIPK9 and CBL3 work together and function in K⺠homeostasis under low-K⺠stress in Arabidopsis.
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MEDLINE
Asunto principal:
Potasio
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Estrés Fisiológico
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Proteínas de Unión al Calcio
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Proteínas Serina-Treonina Quinasas
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Arabidopsis
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Proteínas de Arabidopsis
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Homeostasis
Idioma:
En
Año:
2013
Tipo del documento:
Article