Generation of integration-free neural progenitor cells from cells in human urine.
Nat Methods
; 10(1): 84-9, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-23223155
ABSTRACT
Human neural stem cells hold great promise for research and therapy in neural disease. We describe the generation of integration-free and expandable human neural progenitor cells (NPCs). We combined an episomal system to deliver reprogramming factors with a chemically defined culture medium to reprogram epithelial-like cells from human urine into NPCs (hUiNPCs). These transgene-free hUiNPCs can self-renew and can differentiate into multiple functional neuronal subtypes and glial cells in vitro. Although functional in vivo analysis is still needed, we report that the cells survive and differentiate upon transplant into newborn rat brain.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Orina
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Encéfalo
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Diferenciación Celular
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Ingeniería de Tejidos
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Células Epiteliales
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Reprogramación Celular
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Células-Madre Neurales
Límite:
Animals
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Humans
Idioma:
En
Año:
2013
Tipo del documento:
Article