Purification of A-form DNA fiber samples by the removal of B-form DNA residues.
J Phys Chem B
; 117(6): 1849-56, 2013 Feb 14.
Article
en En
| MEDLINE
| ID: mdl-23330608
ABSTRACT
To date, fiber diffraction on A-form NaDNA has always shown a B-form contamination. Here we have used optic microscopy, calorimetry, and neutron scattering techniques to define a method to obtain DNA fibres samples whose molecules are purely in the A-form. When the impure sample is heated to 320 K, the DNA molecules in the B-form undergo a transition into the A-form. Our studies have modified the accepted phase diagram for NaDNA films by including the dependence of temperature crucial for the purification of A-form samples by removal of B-form contamination.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ADN de Forma A
/
ADN Forma B
Idioma:
En
Año:
2013
Tipo del documento:
Article