Multidimensional protein identification technology for direct-tissue proteomics of heart.
Methods Mol Biol
; 1005: 25-38, 2013.
Article
en En
| MEDLINE
| ID: mdl-23606246
ABSTRACT
Multidimensional protein identification technology (MudPIT) is an invaluable approach to identify proteins at large-scale level. Here, we describe a procedure of investigation to functional characterize the proteomic profile of complex samples such as those from cardiac tissues. In particular, we focus on the main steps concerning sample preparation, MudPIT analysis, tandem mass spectra processing, and biomarker discovery using label-free approaches. Finally, we report a data-derived systems biology approach to identify groups of proteins of over-, under-, and normal expression.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Programas Informáticos
/
Expresión Génica
/
Proteoma
/
Miocardio
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
2013
Tipo del documento:
Article