Spasmolytic effect of curcumin on goat ruminal artery is endothelium independent and by activation of sGC.
Res Vet Sci
; 95(2): 588-93, 2013 Oct.
Article
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| MEDLINE
| ID: mdl-23714042
ABSTRACT
The aim of the present work was to study the mechanism of action of curcumin in vasomotion of a physiologically important artery of ruminant i.e. ruminal artery. ACh and SNP were used to study the role of endothelium in relaxation of this artery. Vasorelaxatation by curcumin was studied in a dose dependent manner, on rings precontracted with 5-hydroxy tryptamine and noradrenalin, in presence and absence of L-NAME, 4AP, ODQ and 4AP+ODQ combination. SNP (1 ηM-100 µM) produced a significant relaxation compared to ACh (0.1-100 µM) on 5-HT (10 µM) and NA (10 µM) induced contraction in endothelium intact rings. Curcumin (10 ηM-100 µM) relaxed the vascular rings in dose dependent manner with maximal relaxation up to 20.94% and 13.81% in 5-HT and NA induced contraction, respectively which was potently blocked by ODQ (10 µM) and combination of 4AP and ODQ (10 µM) but 4AP (10 µM) and L-NAME (100 µM) alone could not block the relaxation and interestingly we observed a slight increase in the tension at higher dose of the agonist (>10 µM). Therefore in goat ruminal artery, curcumin at least in part, act via direct activation of sGC mediated cGMP pathway followed by opening of K(+) ion channel. However other mechanisms may not be ruled out.
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Banco de datos:
MEDLINE
Asunto principal:
Rumen
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Vasoconstricción
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Cabras
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Endotelio Vascular
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Receptores Citoplasmáticos y Nucleares
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Curcumina
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Guanilato Ciclasa
Límite:
Animals
Idioma:
En
Año:
2013
Tipo del documento:
Article