Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells.
Pharmacol Rep
; 65(2): 453-9, 2013.
Article
en En
| MEDLINE
| ID: mdl-23744430
ABSTRACT
BACKGROUND:
Dihydroartemisinin (DHA) exhibits potent anti-malarial and anti-cancer activities. This study aimed to investigate the anti-proliferative effects of a combination of DHA and doxorubicin (DOX) on human breast cancer cells.METHODS:
MTT assay and the combination index (CI) were used to show the anti-proliferative effects and calculate the synergism potential, respectively. Flow cytometry assay was used to detect apoptosis and the intracellular accumulation of DOX. JC-1 staining was used to determine the mitochondrial membrane potential. Western blot analysis was used to detect the protein expression of some apoptosis-related molecules.RESULTS:
Asynergistic anti-proliferative effect was found, and the enhanced anti-cancer activity was observed to be accompanied by the prompt onset of apoptosis in MCF-7 cells. The combinative treatment remarkably decreased the mitochondrial membrane potential and activated caspase cascades more than the mono-treatment. Pretreatment with DHA also did not influence the accumulation of DOX in MCF-7 cells.CONCLUSION:
This study presented a new opportunity to enhance the effectiveness of future treatment regimens of breast cancer using DOX.
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Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Doxorrubicina
/
Apoptosis
/
Artemisininas
Límite:
Female
/
Humans
Idioma:
En
Año:
2013
Tipo del documento:
Article