Changes in the balance between Treg and Th17 cells in patients with chronic hepatitis B.
Diagn Microbiol Infect Dis
; 76(4): 437-44, 2013 Aug.
Article
en En
| MEDLINE
| ID: mdl-23747030
ABSTRACT
The purpose of this study was to explore the role of Treg cells, Th17 cells and cytokines associated with Treg/Th17 differentiation in the occurrence, development and outcome of chronic hepatitis B (CHB). To do so, we detected populations of Treg and Th17 cells and their associated cytokines in the peripheral blood of CHB patients. The populations of Treg cells (CD4(+)CD25(high)CD127(low) T cells) and Th17 cells (CD3(+)CD8(-)IL-17(+) T cells) were analyzed in 46 patients with low to moderate chronic hepatitis B (CHB-LM), 24 patients with severe chronic hepatitis B (CHB-S) and 20 healthy controls (HC) using flow cytometry. The levels of cytokines associated with Treg/Th17 differentiation, including IL-10, TGF-ß1, IL-17 and IL-23, were measured by enzyme-linked immunosorbent assay (ELISA). Our study showed that the imbalance of Treg and Th17 cells might play an important role in the occurrence, development and outcome of CHB.
Palabras clave
ALT; AST; Alanine aminotransferase; Aspartate aminotransferase; CHB; CHB-LM; CHB-S; Chronic hepatitis B; DBil; DC; Dendritic cells; Direct bilirubin; ETV; Entecavir; G-CSF; Granulocyte colony-stimulating factor; HAV; HBV; HBeAg; HBsAg; HC; HCV; HDV; Healthy controls; Hepatitis A virus; Hepatitis B; Hepatitis B e Antigen; Hepatitis B surface; Hepatitis B virus; Hepatitis C virus; Hepatitis D virus; ICAM-1; IL-10; IL-17; Intercellular adhesion molecule-1; Interleukin-17; Ion; Ionomycin; Low to moderate chronic hepatitis B; Mon; Monensin; NF-kappa B; NK; Natural killer; Nuclear factor kappa B; PBMCs; PD-1; PMA; Peripheral blood mononuclear cells; Phorbol 12-myristate-13-acetate; Procedural death factor-1; Severe chronic hepatitis B; T; T helper 17; TBil; TGF-ß1; Th17; Th17 cells; Total bilirubin; Transforming growth factor-ß1; Treg; Treg cells
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Virus de la Hepatitis B
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Citocinas
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Linfocitos T Reguladores
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Hepatitis B Crónica
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Células Th17
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Año:
2013
Tipo del documento:
Article