User guide for mapping-by-sequencing in Arabidopsis.
Genome Biol
; 14(6): R61, 2013 Jun 17.
Article
en En
| MEDLINE
| ID: mdl-23773572
ABSTRACT
Mapping-by-sequencing combines genetic mapping with whole-genome sequencing in order to accelerate mutant identification. However, application of mapping-by-sequencing requires decisions on various practical settings on the experimental design that are not intuitively answered. Following an experimentally determined recombination landscape of Arabidopsis and next generation sequencing-specific biases, we simulated more than 400,000 mapping-by-sequencing experiments. This allowed us to evaluate a broad range of different types of experiments and to develop general rules for mapping-by-sequencing in Arabidopsis. Most importantly, this informs about the properties of different crossing scenarios, the number of recombinants and sequencing depth needed for successful mapping experiments.
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1
Banco de datos:
MEDLINE
Asunto principal:
Mapeo Cromosómico
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Arabidopsis
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Genoma de Planta
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Cromosomas de las Plantas
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Secuenciación de Nucleótidos de Alto Rendimiento
Idioma:
En
Año:
2013
Tipo del documento:
Article