Improved liquid chromatography-tandem mass spectrometric method for the determination of ethyl glucuronide concentrations in hair: applications to forensic cases.
Int J Legal Med
; 128(1): 53-8, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-23824336
ABSTRACT
Ethyl glucuronide (EtG) is a direct marker of ethanol consumption, and its assay in hair is an efficient tool for chronic alcoholism diagnosis. In 2012, the Society of Hair Testing proposed a new consensus for hair concentrations interpretation, strongly advising the use of analytical methods providing a limit of quantification of less than 3 pg/mg. The present work describes the optimization and validation of a previously developed liquid chromatography-tandem mass spectrometric method in order to comply with this recommendation. The concentration range of this improved method is from 3 to 1,000 pg/mg. Some cases are then described to illustrate the usefulness of hair EtG a forensic post-mortem case and two cases of suspension of driving licences. Finally, hair samples of some teetotallers (n = 10) have been analyzed, which allowed neither to quantitate nor to detect any trace of EtG.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Consumo de Bebidas Alcohólicas
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Cromatografía Liquida
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Alcoholismo
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Espectrometría de Masas en Tándem
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Glucuronatos
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Cabello
Tipo de estudio:
Guideline
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article