Bioingeniería; Evolución Molecular Dirigida; Variación Genética; Animales; Clonación Molecular; Biblioteca de Genes; Humanos; Ratones; Mutagénesis Sitio-Dirigida

2'-Deoxy-P-nucleoside-5'-triphosphate; 2'-Deoxyinosine 5'-triphosphate; 7-Deaza-7-(triethylsilylethynyl)deoxyadenosine triphosphate; 8-Oxo-2'-deoxyguanosine-5'-triphosphate; 8-oxo-dGTP; AAV; ABI-REC; Adeno-associated virus; Asymmetric Bridge PCR with Intramolecular Homologous Recombination; Base pairs; CAST; CPEC; CRP; Circular Polymerase Extension Cloning; Combinatorial Active-Site Saturation Test; DGRs; DNA; DNA recombination; Deoxyribonucleic acid; Deoxyribonucleotide triphosphate; Directed evolution; Diversity-generating retroelements; Double-stranded DNA; DuARCheM; Dual Approach to Random Chemical Mutagenesis; EMP; EMS; Enoyl-acyl carrier protein reductase; Error-prone polymerase chain reaction; Error-prone rolling circle amplification; Ethyl methane sulfonate; Exponential Megapriming PCR; GOI; GST; Gene of interest; Genetic diversity; Genome engineering; Glutathione-S-transferase; ITCHY; InDel; Incremental Truncation for the Creation of Hybrid Enzymes; Insertion and deletion; KF; Klenow fragment; MAP; MEGAWHOP; MGS; MLF-SDM; Megaprimed and Ligase-Free PCR-based Method for Site-Directed Mutagenesis; Megaprimer PCR of Whole Plasmid; Metabolic engineering; Mutagenesis Assistant Program; Mutation Generation System; NEB; NRR; New England Biolabs; NiDE; Nicking DNA Endonuclease; Non-homologous random recombination; Nucleotide; OLTA; OSCARR; One-pot Simple Methodology for Casette Randomization and Recombination; OverLap extension PCR and TA cloning; PCR; PCR Production of Circular Plasmid; PERMUTE; PERMutation Using Transposase Engineering; PFLF-MSDM; PLICing; PPCP; PS; PTRec; Phosphorothioate; Phosphorothioate-based DNA Recombination; Phosphorothioate-based Ligase-Independent Gene Cloning; Phosphorylation-Free and Ligase-Free PCR-based Method for Multiple SDM; Polymerase chain reaction; RCA; REs; RF cloning; RGEN; RNA-guided Endonuclease; Random mutagenesis; Restriction enzymes; Restriction-Free cloning; Rolling circle amplification; SDM; SEFC; SHIPREC; SLiCE; SPRINP; STRU-Cloning; SeSaM; Seamless Enzyme-Free Cloning; Seamless Ligation Cloning Extract; Sequence Homology-Independent Protein RECombination; Sequence Saturation Mutagenesis; Single-Primer Reactions In Parallel; Single-Tube Restriction-based Ultrafiltration Cloning; Single-stranded DNA; Site-directed mutagenesis; StEP; Staggered Extension Process; Synthetic biology; T(s); T(v); TALENs; TAM; TIM; TMGS-PCR; TPCR; TRINS; TaGTEAM; Tandem Repeat Insertion; Targeting Glycosylases To Embedded Arrays for Mutagenesis; Transcription activator-like effector nucleases; Transcription-associated mutation; Transfer-PCR; Transitions; Transposon Integration mediated Mutagenesis; Transversions; TriNEx; TriNucleotide EXchange; Truncated Metagenomic Gene-Specific PCR; UDG; USER Friendly DNA Recombination; USERec; Uracil-DNA glycosylase; ZFN; Zinc finger nuclease; bp; cAMP receptor protein; dA(TESE)TP; dITP; dNTP; dPTP; dsDNA; enoyl ACP reductase; epPCR; epRCA; nt; p-Benzoylphenylalanine; pBpa; ssDNA