RNA interference-mediated repression of SmCPS (copalyldiphosphate synthase) expression in hairy roots of Salvia miltiorrhiza causes a decrease of tanshinones and sheds light on the functional role of SmCPS.
Biotechnol Lett
; 36(2): 363-9, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24078134
ABSTRACT
Tanshinones are a group of bioactive abietane-type norditerpenoid quinone compounds in Salvia miltiorrhiza. Copalyldiphosphate synthase of S. miltiorrhiza (SmCPS) is the first key enzyme in tanshinone biosynthesis from the universal diterpene precursor geranylgeranyl diphosphate. Hairy roots of S. miltiorrhiza were transformed with Agrobacterium rhizogenes carrying an RNA interference (RNAi) construct designed to silence SmCPS, and we examined the resulting SmCPS expression and tanshinone accumulation. In SmCPSRNAi hairy roots, the transcript level of SmCPS was reduced to 26 % while the dihydrotanshinone I and cryptotanshinone levels were decreased by 53 and 38 % compared to those of the vector control hairy roots; tanshinone IIA was not detected. Therefore, the decreased expression of SmCPS caused a decrease in tanshinone levels which verifies that SmCPS is a key enzyme for tanshinone biosynthesis in S. miltiorrhiza.
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Banco de datos:
MEDLINE
Asunto principal:
Fenantrenos
/
Proteínas de Plantas
/
Regulación hacia Abajo
/
Transferasas Alquil y Aril
/
Salvia miltiorrhiza
/
Interferencia de ARN
/
Diterpenos
Tipo de estudio:
Etiology_studies
Idioma:
En
Año:
2014
Tipo del documento:
Article