[Study of the mRNA-binding region of ribosomes at different steps of translation. II. Affinity modification of Escherichia coli ribosomes by benzylidene derivative of AUGU6 in the 70S initiation complex]. / Issledovanie mRNK-sviazyvaiushchei ioblasti ribosomy na raznykh étapakh transliatsii. II. Affinnaia modifikatsiia ribosom Escherichia coli benzilidenovym proizvodnym AUGU6 v 70S komplekse initsiatsii.
Mol Biol (Mosk)
; 19(4): 1079-85, 1985.
Article
en Ru
| MEDLINE
| ID: mdl-2413345
ABSTRACT
2',3'-O-(4-[N-(2-chloroethyl)-N-methylamino]) benzylidene derivative of AUGU6 was used for identification of the proteins in the region of the mRNA-binding centre of E. coli ribosomes. This derivative alkylated ribosomes (preferentially 30S ribosomal) with high efficiency within the 70S initiation complex. In both 30S and 50S ribosomal subunits proteins and rRNA were modified. Specificity of the alkylation of ribosomal proteins and rRNA with the reagent was proved by the inhibitory action of AUGU6. Using the method of two-dimensional electrophoresis in polyacrylamide gel the proteins S4, S12, S13, S14, S15, S18, S19 and S20/L26 which are labelled by the analog of mRNA were identified.
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Banco de datos:
MEDLINE
Asunto principal:
Oligorribonucleótidos
/
Iniciación de la Cadena Peptídica Traduccional
/
Ribosomas
/
Compuestos de Bencilideno
/
ARN Bacteriano
/
ARN Mensajero
/
Escherichia coli
Tipo de estudio:
Prognostic_studies
Idioma:
Ru
Año:
1985
Tipo del documento:
Article