Effect of lipid composition of cationic SUV liposomes on materno-fetal transfer of warfarin across the perfused human term placenta.
Placenta
; 34(12): 1216-22, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-24183755
ABSTRACT
INTRODUCTION:
Use of drugs that cross the placenta freely are currently avoided during pregnancy. We investigated whether cationic small unilamellar (SUV) liposomes of different lipid compositions could prevent the transfer and uptake of warfarin across human term placenta.METHODS:
Cationic liposomes encapsulated warfarin was prepared by using lecithin (F-SUV) or sterylamine (S-SUV) with cholesterol and stearylamine. The size distribution, encapsulation efficiency, and stability were determined in blood-based media. The transfer kinetics of free and liposomally encapsulated warfarin were studied in a dually perfused isolated lobule of human term placenta with creatinine. Concentrations of warfarin were measured by fluorimetry. Data are expressed as % of initial dose added and given as mean ± sd.RESULTS:
Warfarin crossed the placenta freely (14.9 ± 1.1%). Trans placental transfer of warfarin was significantly reduced by F-SUV (6.4 ± 0.6%; P < 0.001) and S-SUV liposomes (5.0 ± 0.8%; P < 0.001). Placental uptake of F-SUV (6.3 ± 1.7%; P < 0.001) was greater than that of S-SUV liposomes (2.2 ± 0.5%; P < 0.001).CONCLUSION:
Our data suggest that cationic liposomes reduce trans placental transfer of warfarin. If confirmed "in vivo", liposomes might provide an alternative non-invasive method of drug delivery to the mother.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Placenta
/
Warfarina
/
Sistemas de Liberación de Medicamentos
/
Lípidos
/
Intercambio Materno-Fetal
/
Anticoagulantes
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Año:
2013
Tipo del documento:
Article