Multiplex minisequencing screening for PTC genotype associated with bitter taste perception.
Mol Biol Rep
; 41(3): 1563-7, 2014 Mar.
Article
en En
| MEDLINE
| ID: mdl-24413990
ABSTRACT
Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Feniltiourea
/
Gusto
/
Receptores Acoplados a Proteínas G
/
Percepción del Gusto
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
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Screening_studies
Límite:
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article