Infectivity and complete nucleotide sequence of cucumber fruit mottle mosaic virus isolate Cm cDNA.
Arch Virol
; 159(7): 1807-11, 2014 Jul.
Article
en En
| MEDLINE
| ID: mdl-24473709
ABSTRACT
Three isolates of cucumber fruit mottle mosaic virus (CFMMV) were collected from melon, cucumber, and pumpkin plants in Korea. A full-length cDNA clone of CFMMV-Cm (melon isolate) was produced and evaluated for infectivity after T7 transcription in vitro (pT7CF-Cmflc). The complete CFMMV genome sequence of the infectious clone pT7CF-Cmflc was determined. The genome of CFMMV-Cm consisted of 6,571 nucleotides and shared high nucleotide sequence identity (98.8 %) with the Israel isolate of CFMMV. Based on the infectious clone pT7CF-Cmflc, a CaMV 35S-promoter driven cDNA clone (p35SCF-Cmflc) was subsequently constructed and sequenced. Mechanical inoculation with RNA transcripts of pT7CF-Cmflc and agro-inoculation with p35SCF-Cmflc resulted in systemic infection of cucumber and melon, producing symptoms similar to those produced by CFMMV-Cm. Progeny virus in infected plants was detected by RT-PCR, western blot assay, and transmission electron microscopy.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
Tobamovirus
/
ADN Complementario
/
Cucurbitaceae
Idioma:
En
Año:
2014
Tipo del documento:
Article