Lipopolysaccharide impairs insulin sensitivity via activation of phosphoinositide 3-kinase in adipocytes.
Immunopharmacol Immunotoxicol
; 36(2): 145-9, 2014 Apr.
Article
en En
| MEDLINE
| ID: mdl-24506665
ABSTRACT
The effect of lipopolysaccharide (LPS) on insulin sensitivity in adipocytes were examined by using differentiated 3T3-L1 adipocytes. Insulin-mediated activation of insulin receptor substrate (IRS) 1/2 was inhibited in LPS-pretreated adipocytes and IRS1/2-mediated Akt activation was also attenuated in those cells. LPS inhibited activation of glycogen synthase kinase 3 as a negative regulator of glycogenesis and impaired the glycogen synthesis in response to insulin. LPS-induced activation of phosphoinositide 3-kinase (PI3K) in adipocytes. Involvement of suppressor of cytokine signaling 3 (SOCS3) in LPS-induced IRS1/2 inhibition was excluded. Considering that both insulin and LPS were able to activate the PI3K/Akt signaling pathway, LPS was suggested to impair insulin sensitivity of adipocytes through down-regulating insulin-mediated PI3K/Akt activation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Resistencia a la Insulina
/
Lipopolisacáridos
/
Adipocitos
/
Fosfatidilinositol 3-Quinasas
/
Proteínas Proto-Oncogénicas c-akt
/
Insulina
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article