BSH-CP based 3D solid-state NMR experiments for protein resonance assignment.
J Biomol NMR
; 59(1): 15-22, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24584701
ABSTRACT
We have recently presented band-selective homonuclear cross-polarization (BSH-CP) as an efficient method for CO-CA transfer in deuterated as well as protonated solid proteins. Here we show how the BSH-CP CO-CA transfer block can be incorporated in a set of three-dimensional (3D) solid-state NMR (ssNMR) pulse schemes tailored for resonance assignment of proteins at high static magnetic fields and moderate magic-angle spinning rates. Due to the achieved excellent transfer efficiency of 33 % for BSH-CP, a complete set of 3D spectra needed for unambiguous resonance assignment could be rapidly recorded within 1 week for the model protein ubiquitin. Thus we expect that BSH-CP could replace the typically used CO-CA transfer schemes in well-established 3D ssNMR approaches for resonance assignment of solid biomolecules.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas
/
Resonancia Magnética Nuclear Biomolecular
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2014
Tipo del documento:
Article