Backscattered electron SEM imaging of cells and determination of the information depth.
J Microsc
; 254(2): 75-83, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24650037
ABSTRACT
Backscattered electron imaging of HT29 colon carcinoma cells in a scanning electron microscope was studied. Thin cell sections were placed on indium-tin-oxide-coated glass slides, which is a promising substrate material for correlative light and electron microscopy. The ultrastructure of HT29 colon carcinoma cells was imaged without poststaining by exploiting the high chemical sensitivity of backscattered electrons. Optimum primary electron energies for backscattered electron imaging were determined which depend on the section thickness. Charging effects in the vicinity of the SiO2 nanoparticles contained in cell sections could be clarified by placing cell sections on different substrates. Moreover, a method is presented for information depth determination of backscattered electrons which is based on the imaging of subsurface nanoparticles embedded by the cells.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microscopía Electrónica de Rastreo
Límite:
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article