ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle.
Mol Biochem Parasitol
; 193(2): 110-3, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24681203
ABSTRACT
Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Trypanosoma cruzi
/
Cromatina
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Proteínas de Ciclo Celular
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Complejo de Reconocimiento del Origen
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Componente 7 del Complejo de Mantenimiento de Minicromosoma
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article