4-1BB-based isolation and expansion of CD8+ T cells specific for self-tumor and non-self-tumor antigens for adoptive T-cell therapy.
J Immunother
; 37(4): 225-36, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24714356
ABSTRACT
Adoptive T-cell therapy is a promising approach to the immunotherapy of cancer, but for it to be a general cancer therapy a simple and standardized procedure for producing tumor-specific CD8 T cells is needed. On the basis of a unique property of 4-1BB (CD137), the selective expression on activated T cells, we have developed a simple and practical protocol to produce antigen-specific CD8 T cells from peripheral blood mononuclear cells. We have proved the feasibility of this procedure by isolating and expanding cytomegalovirus-specific CD8 T cells, and applied the procedure to produce Epstein-Barr virus (EBV)-specific CD8 T cells. By using this procedure, we could readily produce 10-10 antigen-specific CD8 T cells from 30 to 50 mL of blood in about 4 weeks. Moreover, our protocol allowed us to produce, from solid cancer patients, CD8 T cells that were specific for self/tumor antigens such as human telomerase reverse transcriptase (hTERT). It is interesting to note that, we were unable to amplify hTERT-specific CD8 T cells from healthy donors. Our protocol can be readily translated into cGMP-compliant production and is currently being used to produce EBV-specific CD8 T cells for phase I clinical trial. We believe that our method will provide a practical and effective option for adoptive T-cell therapy in the clinic.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Autoantígenos
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Inmunoterapia Adoptiva
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Linfocitos T CD8-positivos
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Miembro 9 de la Superfamilia de Receptores de Factores de Necrosis Tumoral
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Antígenos de Neoplasias
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Neoplasias
Tipo de estudio:
Guideline
Límite:
Animals
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Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article