Rapid adaptive optical recovery of optimal resolution over large volumes.
Nat Methods
; 11(6): 625-8, 2014 Jun.
Article
en En
| MEDLINE
| ID: mdl-24727653
ABSTRACT
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
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1
Banco de datos:
MEDLINE
Asunto principal:
Microscopía Confocal
/
Óptica y Fotónica
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article