Catalase prevents lipid peroxidation and enhances survival of caprine preantral follicles cryopreserved in a 1,2-propanediol-freezing medium.
Biopreserv Biobank
; 10(4): 338-42, 2012 Aug.
Article
en En
| MEDLINE
| ID: mdl-24849881
ABSTRACT
The objectives of this study were to determine 1) the optimal concentration (1.0 or 1.5 M) and duration of exposure (5, 10, or 20 min) of ovarian tissue to 1,2-propanediol (PROH) on morphology and viability of caprine preantral follicles; and 2) the effect of supplementing cryopreservation medium supplementation with Trolox(®) (0.1, 0.5, or 1.0 mM) or catalase (5, 10, or 20 IU/mL) on follicular morphology, viability, and lipid peroxidation. Cryopreservation decreased (p<0.05) percentages of normal follicles relative to the control (84%). Although supplementation of the cryopreservation medium (1.0 M PROH) with catalase (10 or 20 IU/mL) or Trolox(®) (0.1 mM) resulted in follicular morphology and viability similar to that in the controls (P>0.05), lipid peroxidation was reduced only when 20 IU/mL catalase was added to the cryopreservation medium.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Catalasa
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Criopreservación
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Propilenglicol
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Crioprotectores
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Congelación
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Folículo Ovárico
Límite:
Animals
Idioma:
En
Año:
2012
Tipo del documento:
Article