Dolichol biosynthesis: the occurrence of epoxy dolichol in skipjack tuna liver.
Biochem Biophys Res Commun
; 453(2): 277-81, 2014 Oct 17.
Article
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| MEDLINE
| ID: mdl-24866245
ABSTRACT
Polyisoprenoid alcohols from the livers of temperate sea fish (skipjack tuna, chub mackerel, red sea bream and rainbow trout) were analyzed by using 2D-TLC, electrospray ionization (ESI) mass spectrometry and NMR methods. Dolichols (Dols) were detected in all the fish livers, and they were composed of 19-22 isoprene units with Dol-20 as the predominant prenolog. In addition, Dol-like family compounds were found by using 2D-TLC on skipjack tuna samples. These compounds were found to have a larger molecular mass than the Dol family by 16 mass units. NMR analysis indicated that the Dol-like compounds were consistent with the terminal epoxide structure of Dols (the ω-oxirane derivatives of Dols). ESI analysis also revealed the occurrence of dehydro molecules in both Dols and epoxy Dols (Dol-like) fractions. The occurrence of epoxy Dols in fish is discussed in context with the biosynthesis of Dols, which is responsible for forming Dol phosphate, which lead to Dol-PP-oligosaccharide.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Atún
/
Dolicoles
/
Hígado
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article