RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination.
Genes Dev
; 28(11): 1239-51, 2014 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-24888591
ABSTRACT
RNA-binding protein CsrA is a key regulator of a variety of cellular processes in bacteria, including carbon and stationary phase metabolism, biofilm formation, quorum sensing, and virulence gene expression in pathogens. CsrA binds to bipartite sequence elements at or near the ribosome loading site in messenger RNA (mRNA), most often inhibiting translation initiation. Here we describe an alternative novel mechanism through which CsrA achieves negative regulation. We show that CsrA binding to the upstream portion of the 5' untranslated region of Escherichia coli pgaA mRNA-encoding a polysaccharide adhesin export protein-unfolds a secondary structure that sequesters an entry site for transcription termination factor Rho, resulting in the premature stop of transcription. These findings establish a new paradigm for bacterial gene regulation in which remodeling of the nascent transcript by a regulatory protein promotes Rho-dependent transcription attenuation.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Factor Rho
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ARN Bacteriano
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Regulación Bacteriana de la Expresión Génica
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Proteínas de Unión al ARN
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Salmonella enterica
Idioma:
En
Año:
2014
Tipo del documento:
Article