Heterogeneity of the internal transcribed spacer region in Leishmania tropica isolates from southern Iran.
Exp Parasitol
; 144: 44-51, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-24932536
ABSTRACT
Most of cutaneous leishmaniasis cases occur in only 7 countries, including Iran. Leishmania tropica is the main cause of anthroponotic cutaneous leishmaniasis in Iran. In order to study the heterogeneity and phylogeny of L. tropica in southern Iran, a total of 61 isolates were obtained from Bam district and the cities Kerman and Shiraz. The internal transcribed spacer (ITS) from the ribosomal DNA locus was amplified and then analysed by sequencing. Analysis of the ITS sequences showed four haplotypes in the isolates, including 3 haplotypes among the 58 isolates from the south eastern region, including Bam district and Kerman city, and 2 haplotypes among the 3 isolates from Shiraz city. The results showed a monophyletic structure for the south eastern population. In comparison to GenBank sequences of L. tropica from different countries, most of the southeast Iranian and Indian isolates are comprised in one cluster, while isolates from other countries and few other Iranian isolates group in a different cluster. Analysis of ITS sequences of south eastern L. tropica showed a homogeneous population which could be the basis for other molecular epidemiology studies using more discriminative markers and tracing possible changes in the population structure of L. tropica.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Leishmania tropica
/
ADN Protozoario
/
Leishmaniasis Cutánea
/
Heterogeneidad Genética
/
ADN Espaciador Ribosómico
Límite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Humans
/
Infant
/
Middle aged
País como asunto:
Asia
Idioma:
En
Año:
2014
Tipo del documento:
Article