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Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells.
Gildea, John J; Seaton, Joscelyn E; Victor, Ken G; Reyes, Camellia M; Bigler Wang, Dora; Pettigrew, Abigail C; Courtner, Crystal E; Shah, Neema; Tran, Hanh T; Van Sciver, Robert E; Carlson, Julia M; Felder, Robin A.
  • Gildea JJ; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA. Electronic address: jjg5b@virginia.edu.
  • Seaton JE; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Victor KG; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Reyes CM; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Bigler Wang D; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Pettigrew AC; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Courtner CE; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Shah N; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Tran HT; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Van Sciver RE; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Carlson JM; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
  • Felder RA; The University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Clin Biochem ; 47(15): 89-94, 2014 Oct.
Article en En | MEDLINE | ID: mdl-24976626
ABSTRACT

OBJECTIVES:

Exosomes are 50-90nm extracellular membrane particles that may mediate trans-cellular communication between cells and tissues. We have reported that human urinary exosomes contain miRNA that are biomarkers for salt sensitivity and inverse salt sensitivity of blood pressure. This study examines exosomal transfer between cultured human renal proximal tubule cells (RPTCs) and from RPTCs to human distal tubule and collecting duct cells. DESIGN AND

METHODS:

For RPTC-to-RPTC exosomal transfer, we utilized 5 RPTC lines producing exosomes that were fluorescently labeled with exosomal-specific markers CD63-EGFP or CD9-RFP. Transfer between RPTCs was demonstrated by co-culturing CD63-EGFP and CD9-RFP stable clones and performing live confocal microscopy. For RPTC-to-distal segment exosomal transfer, we utilized 5 distal tubule and 3 collecting duct immortalized cell lines.

RESULTS:

Time-lapse videos revealed unique proximal tubule cellular uptake patterns for exosomes and eventual accumulation into the multivesicular body. Using culture supernatant containing exosomes from 3 CD9-RFP and 2 CD63-EGFP RPTC cell lines, all 5 distal tubule cell lines and all 3 collecting duct cell lines showed exosomal uptake as measured by microplate fluorometry. Furthermore, we found that RPTCs stimulated with fenoldopam (dopamine receptor agonist) had increased production of exosomes, which upon transfer to distal tubule and collecting duct cells, reduced the basal reactive oxygen species (ROS) production rates in those recipient cells.

CONCLUSION:

Due to the complex diversity of exosomal contents, this proximal-to-distal vesicular inter-nephron transfer may represent a previously unrecognized trans-renal communication system.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Exosomas / Túbulos Renales Colectores / Túbulos Renales Distales / Túbulos Renales Proximales Límite: Humans Idioma: En Año: 2014 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Exosomas / Túbulos Renales Colectores / Túbulos Renales Distales / Túbulos Renales Proximales Límite: Humans Idioma: En Año: 2014 Tipo del documento: Article