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Preparation of polyphosphazene hydrogels for enzyme immobilization.
Qian, Yue-Cheng; Chen, Peng-Cheng; He, Gui-Jin; Huang, Xiao-Jun; Xu, Zhi-Kang.
  • Qian YC; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. education@zju.edu.cn.
  • Chen PC; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. chenpengcheng@zju.edu.cn.
  • He GJ; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. guikinghe@zju.edu.cn.
  • Huang XJ; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. hxjzxh@zju.edu.cn.
  • Xu ZK; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. xuzk@zju.edu.cn.
Molecules ; 19(7): 9850-63, 2014 Jul 08.
Article en En | MEDLINE | ID: mdl-25006790
ABSTRACT
We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Compuestos Organofosforados / Polímeros / Hidrogeles / Enzimas Inmovilizadas Idioma: En Año: 2014 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Compuestos Organofosforados / Polímeros / Hidrogeles / Enzimas Inmovilizadas Idioma: En Año: 2014 Tipo del documento: Article