Independent origin of plasmodium falciparum antifolate super-resistance, Uganda, Tanzania, and Ethiopia.
Emerg Infect Dis
; 20(8): 1280-6, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-25061906
ABSTRACT
Super-resistant Plasmodium falciparum threatens the effectiveness of sulfadoxine-pyrimethamine in intermittent preventive treatment for malaria during pregnancy. It is characterized by the A581G Pfdhps mutation on a background of the double-mutant Pfdhps and the triple-mutant Pfdhfr. Using samples collected during 2004-2008, we investigated the evolutionary origin of the A581G mutation by characterizing microsatellite diversity flanking Pfdhps triple-mutant (437G+540E+581G) alleles from 3 locations in eastern Africa and comparing it with double-mutant (437G+540E) alleles from the same area. In Ethiopia, both alleles derived from 1 lineage that was distinct from those in Uganda and Tanzania. Uganda and Tanzania triple mutants derived from the previously characterized southeastern Africa double-mutant lineage. The A581G mutation has occurred multiple times on local Pfdhps double-mutant backgrounds; however, a novel microsatellite allele incorporated into the Tanzania lineage since 2004 illustrates the local expansion of emergent triple-mutant lineages.
Palabras clave
581; Dihydrofolate reductase; Ethiopia; Pfdhfr; Pfdhps; Plasmodium falciparum; Tanzania; Uganda; dihydropteroate synthetase; eastern Africa; intermittent preventive treatment in pregnant women; malaria; microsatellite; parasites; resistance; single-nucleotide polymorphism; sulfadoxine-pyrimethamine; vector-borne infections
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Plasmodium falciparum
/
Resistencia a Medicamentos
/
Malaria Falciparum
/
Antagonistas del Ácido Fólico
Límite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Humans
/
Infant
País como asunto:
Africa
Idioma:
En
Año:
2014
Tipo del documento:
Article