A procedure to obtain long-lasting fluorescence in formaldehyde fixed tissues.
Virchows Arch A Pathol Anat Histopathol
; 415(5): 391-3, 1989.
Article
en En
| MEDLINE
| ID: mdl-2508300
ABSTRACT
The authors made a study of the small intestine by immunofluorescence in 5% phosphate-buffered formaldehyde fixed tissues, using the Nairn (1976) technique associated with Evans blue (Fry and Wilkinson 1963). The ability of the sections to fluoresce has been maintained after a storage time of more than 4 years. Similar very good results were obtained when skin biopsies from pemphigus foliaceus patients were fixed in formaldehyde for two h and afterwards washed in 30% sucrose solution for 12 to 18 h.
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Banco de datos:
MEDLINE
Asunto principal:
Piel
/
Inmunoglobulina A
/
Inmunoglobulina G
/
Pénfigo
/
Mucosa Intestinal
Límite:
Humans
Idioma:
En
Año:
1989
Tipo del documento:
Article