Isolation of arginine kinase from Apis cerana cerana and its possible involvement in response to adverse stress.
Cell Stress Chaperones
; 20(1): 169-83, 2015 Jan.
Article
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| MEDLINE
| ID: mdl-25135575
ABSTRACT
Arginine kinases (AK) in invertebrates play the same role as creatine kinases in vertebrates. Both proteins are important for energy metabolism, and previous studies on AK focused on this attribute. In this study, the arginine kinase gene was isolated from Apis cerana cerana and was named AccAK. A 5'-flanking region was also cloned and shown to contain abundant putative binding sites for transcription factors related to development and response to adverse stress. We imitated several abiotic and biotic stresses suffered by A. cerana cerana during their life, including heavy metals, pesticides, herbicides, heat, cold, oxidants, antioxidants, ecdysone, and Ascosphaera apis and then studied the expression patterns of AccAK after these treatments. AccAK was upregulated under all conditions, and, in some conditions, this response was very pronounced. Western blot and AccAK enzyme activity assays confirmed the results. In addition, a disc diffusion assay showed that overexpression of AccAK reduced the resistance of Escherichia coli cells to multiple adverse stresses. Taken together, our results indicated that AccAK may be involved of great significance in response to adverse abiotic and biotic stresses.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Arginina Quinasa
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Estrés Fisiológico
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Abejas
Límite:
Animals
Idioma:
En
Año:
2015
Tipo del documento:
Article