[HPLC fingerprint of Callicarpa nudiflora].

ABSTRACT

OBJECTIVE: To establish HPLC fingerprint method for the quality evaluation and discrimination of Callicarpa nudiflora. METHODS: The chromatographic fingerprint of the 12 samples was determined by injecting 20 microL of the sample solution each time on a reversed phase Agilent TC-C18 column with the gradient elution solvent system composed of 0.30% HAc solution and acetonitrile. The flow rate was 1.0 mL/min, the column temperature was maintained at 30 degrees C and the detection wavelength was set at 254 nm. RESULTS: The HPLC fingerprint of Callicarpa nudiflora was set up,which included 20 common peaks, and the similarities of 12 samples were basically more than 0.90. Also,3 components were identified. CONCLUSION: The method is accurate, repeatable, reliable and can fully reflect the chemical composition information, which can be used for the discrimination and the quality control of Callicarpa nudiflora.