Dynamic analysis of CO2 labeling and cell respiration using membrane-inlet mass spectrometry.
Methods Mol Biol
; 1191: 175-94, 2014.
Article
en En
| MEDLINE
| ID: mdl-25178791
ABSTRACT
Here, we introduce a mass spectrometry-based analytical method and relevant technical details for dynamic cell respiration and CO2 labeling analysis. Such measurements can be utilized as additional information and constraints for model-based (13)C metabolic flux analysis. Dissolved dynamics of oxygen consumption and CO2 mass isotopomer evolution from (13)C-labeled tracer substrates through different cellular processes can be precisely measured on-line using a miniaturized reactor system equipped with a membrane-inlet mass spectrometer. The corresponding specific rates of physiologically relevant gases and CO2 mass isotopomers can be quantified within a short-term range based on the liquid-phase dynamics of dissolved fermentation gases.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Consumo de Oxígeno
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Espectrometría de Masas
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Dióxido de Carbono
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Respiración de la Célula
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Análisis de Flujos Metabólicos
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Modelos Biológicos
Idioma:
En
Año:
2014
Tipo del documento:
Article