mRNA expression and localization of LPS-induced ß-defensin isoforms in rat salivary glands.
Bull Tokyo Dent Coll
; 55(3): 139-47, 2014.
Article
en En
| MEDLINE
| ID: mdl-25212559
ABSTRACT
ß-defensins are small, cationic peptides with broad-spectrum antimicrobial activity that are produced by mucosal epithelia. However, little is known about the expression of ß-defensins in the major salivary glands. The purpose of this study was to characterize expression of rat ß-defensin-1 (RBD-1) and -2 (RBD-2) mRNA within the major salivary glands together with the effect of injection of intraductal lipopolysaccharide (LPS) on that expression. ß-defensin mRNA expression was quantitated by RT-PCR in salivary gland tissues and salivary acinar and striated duct cells collected by laser captured microdissection. RBD-1 and -2 were expressed in the parotid gland, the submandibular gland, and the sublingual gland. ß-defensins were expressed in both the acinar and striated duct cells of the major salivary glands. Intraductal injection of LPS increased expression of RBD-1 and -2 mRNA, which peaked at 12 hrs. These results suggest that salivary cells (acinar and striated duct cells) have the potential to produce ß-defensins.
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Banco de datos:
MEDLINE
Asunto principal:
Glándulas Salivales
/
ARN Mensajero
/
Lipopolisacáridos
/
Beta-Defensinas
Límite:
Animals
Idioma:
En
Año:
2014
Tipo del documento:
Article