Freezing and storage at -20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis.
Diagn Microbiol Infect Dis
; 80(3): 197-9, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-25241642
ABSTRACT
Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR. No significant differences were found depending on duration or storage conditions at -20 °C or -80 °C.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Manejo de Especímenes
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Toxoplasma
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Toxoplasmosis
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ADN Protozoario
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Técnicas de Diagnóstico Molecular
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Congelación
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Idioma:
En
Año:
2014
Tipo del documento:
Article