Effects of microbubble size on ultrasound-mediated gene transfection in auditory cells.
Biomed Res Int
; 2014: 840852, 2014.
Article
en En
| MEDLINE
| ID: mdl-25254216
ABSTRACT
Gene therapy for sensorineural hearing loss has recently been used to insert genes encoding functional proteins to preserve, protect, or even regenerate hair cells in the inner ear. Our previous study demonstrated a microbubble- (MB-)facilitated ultrasound (US) technique for delivering therapeutic medication to the inner ear. The present study investigated whether MB-US techniques help to enhance the efficiency of gene transfection by means of cationic liposomes on HEI-OC1 auditory cells and whether MBs of different sizes affect such efficiency. Our results demonstrated that the size of MBs was proportional to the concentration of albumin or dextrose. At a constant US power density, using 0.66, 1.32, and 2.83 µm albumin-shelled MBs increased the transfection rate as compared to the control by 30.6%, 54.1%, and 84.7%, respectively; likewise, using 1.39, 2.12, and 3.47 µm albumin-dextrose-shelled MBs increased the transfection rates by 15.9%, 34.3%, and 82.7%, respectively. The results indicate that MB-US is an effective technique to facilitate gene transfer on auditory cells in vitro. Such size-dependent MB oscillation behavior in the presence of US plays a role in enhancing gene transfer, and by manipulating the concentration of albumin or dextrose, MBs of different sizes can be produced.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Terapia Genética
/
Técnicas de Transferencia de Gen
/
Pérdida Auditiva Sensorineural
Límite:
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article