[Construction of prokaryotic expression vector for Ag85A-HA2 fusion gene and studies on the immunity efficacy of fusion protein against influenza A virus].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 45(4): 547-51, 2014 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-25286673
ABSTRACT
OBJECTIVE:
To construct Ag85A-HA2 prokaryotic expression vector, express the fusion protein and study the immunity efficacy of fusion protein against influenza A virus.METHODS:
Ag85A-HA2 prokaryotic expression vector was constructed and induced with IPTG. The fusion protein was identified by SDS-PAGE and purified with His-Tag affinity chromatography. The BALB/c mice were immunized with fusion protein. Then the pathological section, lung index, lung inhibitory rate and death-protection rate were tested to evaluate the immunity efficacy of fusion protein.RESULTS:
pET-32a(+)/Ag85A-HA2 prokaryotic expression vector was constructed successfully. And SDS-PAGE indicated that fusion protein was expressed correctly with a molecular mass of 70 x 10(3). The lung index and death-protection rate in experimental group were 39.30% and 80%, higher than that of control group. The pathological section also demonstrated that Ag85A-HA2 fusion protein had a protective effect on murine lungs.CONCLUSION:
Ag85A-HA2 prokaryotic expression vector was successfully constructed, inducible expression and the fusion protein had an immunity efficacy against influenza A virus in animal experiment.
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Banco de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
/
Proteínas Recombinantes de Fusión
/
Vacunas contra la Influenza
/
Infecciones por Orthomyxoviridae
Límite:
Animals
Idioma:
Zh
Año:
2014
Tipo del documento:
Article