High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories.
Opt Express
; 22(20): 24224-34, 2014 Oct 06.
Article
en En
| MEDLINE
| ID: mdl-25321997
ABSTRACT
A simple beam-scanning optical design based on Lissajous trajectory imaging is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In brief, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Since sub-trajectory and full-trajectory imaging are simply different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired. The optical hardware required to perform Lissajous imaging represents only a minor modification to established beam-scanning hardware, combined with additional control and data acquisition electronics. Preliminary studies based on laser transmittance imaging and polarization-dependent second harmonic generation microscopy support the viability of the approach both for detection of subtle changes in large signals and for trace-light detection of transient fluctuations.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Algoritmos
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Procesamiento de Imagen Asistido por Computador
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Diagnóstico por Imagen
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Fantasmas de Imagen
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Microscopía de Polarización
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Modelos Teóricos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article