Analysis of (dichloromethylene) bisphosphonate in urine by capillary gas chromatography-mass spectrometry.
J Pharm Biomed Anal
; 7(12): 1623-9, 1989.
Article
en En
| MEDLINE
| ID: mdl-2535209
ABSTRACT
An anion exchange extraction method of bisphosphonates from urine is described. More than 90% of the (dichloromethylene) bisphosphonate (Cl2MBP, clodronate) was recovered from urine. The extracted bisphosphonates were trimethylsilylated and analysed with capillary gas chromatography-mass spectrometry (GC/MS). The mass spectrometric techniques used were electron ionization (EI), ammonia chemical ionization (CI), ammonia CI tandem mass spectrometry and methane negative chemical ionization (NCI). The limit of detection of Cl2MBP was 25 pg/injection in the NCI/selective ion recording (SIR)-mode. At 100 ng ml-1 of Cl2MBP the precision of the whole assay method was 17.9% (N = 6). The NCI/SIR technique offers a sensitive and highly selective method for the quantitation of Cl2MBP in urine.
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Banco de datos:
MEDLINE
Asunto principal:
Ácido Clodrónico
Límite:
Humans
Idioma:
En
Año:
1989
Tipo del documento:
Article