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Attenuated West Nile virus mutant NS1130-132QQA/175A/207A exhibits virus-induced ultrastructural changes and accumulation of protein in the endoplasmic reticulum.
Whiteman, Melissa C; Popov, Vsevolod; Sherman, Michael B; Wen, Julie; Barrett, Alan D T.
  • Whiteman MC; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA.
  • Popov V; Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA.
  • Sherman MB; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA.
  • Wen J; Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
  • Barrett AD; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas, USA Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA Institu
J Virol ; 89(2): 1474-8, 2015 Jan 15.
Article en En | MEDLINE | ID: mdl-25392222
ABSTRACT
We have previously shown that ablation of the three N-linked glycosylation sites in the West Nile virus NS1 protein completely attenuates mouse neuroinvasiveness (≥1,000,000 PFU). Here, we compared the replication of the NS1130-132QQA/175A/207A mutant to that of the parental NY99 strain in monkey kidney Vero cells. The results suggest that the mechanism of attenuation is a lack of NS1 glycosylation, which blocks efficient replication, maturation, and NS1 secretion from the endoplasmic reticulum and results in changes to the virus-induced ultrastructure.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Replicación Viral / Virus del Nilo Occidental / Orgánulos / Proteínas no Estructurales Virales / Sustitución de Aminoácidos / Retículo Endoplásmico Límite: Animals Idioma: En Año: 2015 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Replicación Viral / Virus del Nilo Occidental / Orgánulos / Proteínas no Estructurales Virales / Sustitución de Aminoácidos / Retículo Endoplásmico Límite: Animals Idioma: En Año: 2015 Tipo del documento: Article