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Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats.
Fischer, Melina; Freuling, Conrad M; Müller, Thomas; Schatz, Juliane; Rasmussen, Thomas Bruun; Chriel, Mariann; Balkema-Buschmann, Anne; Beer, Martin; Hoffmann, Bernd.
  • Fischer M; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. mlnfis@aol.com.
  • Freuling CM; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Conrad.Freuling@fli.bund.de.
  • Müller T; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Thomas.Muller@fli.bund.de.
  • Schatz J; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. julianeschatz@hotmail.com.
  • Rasmussen TB; DTU Vet, Technical University of Denmark, Lindholm, DK-4771, Kalvehave, Denmark. tbrur@vet.dtu.dk.
  • Chriel M; DTU Vet, Technical University of Denmark, DK-1870, Frederiksberg C, Denmark. march@vet.dtu.dk.
  • Balkema-Buschmann A; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. anne.balkema-buschmann@fli.bund.de.
  • Beer M; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Martin.Beer@fli.bund.de.
  • Hoffmann B; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany. Bernd.Hoffmann@fli.bund.de.
Virol J ; 11: 196, 2014 Nov 25.
Article en En | MEDLINE | ID: mdl-25420461
ABSTRACT

BACKGROUND:

In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR).

FINDINGS:

In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences.

CONCLUSIONS:

Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Orofaringe / Rhabdoviridae / Quirópteros / Infecciones por Rhabdoviridae Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País como asunto: Europa Idioma: En Año: 2014 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Orofaringe / Rhabdoviridae / Quirópteros / Infecciones por Rhabdoviridae Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País como asunto: Europa Idioma: En Año: 2014 Tipo del documento: Article