(68)Ga-AMBA and (18)F-FDG for preclinical PET imaging of breast cancer: effect of tamoxifen treatment on tracer uptake by tumor.
Nucl Med Biol
; 42(2): 92-8, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25459112
ABSTRACT
INTRODUCTION:
AMBA is a bombesin analogue that binds to GRPr. In a mouse model of estrogen-dependent human breast cancer, we tested whether (68)Ga-AMBA can be used for PET detection of GRPr-expressing tumors and could be more accurate than (18)F-FDG to monitor tumor response to hormone therapy.METHODS:
The radiolabeling of (68)Ga-AMBA was automated using a R&D Synchrom module. ZR75-1, a breast cancer cell line, was xenografted in nude mice. (68)Ga-AMBA tumor uptake was compared with that of (18)F-FDG before and after treatment with tamoxifen.RESULTS:
AMBA was (68)Ga-radiolabelled in 30min with 95.3% yield and purity≥98%. Prior to treatment, (68)Ga-AMBA was highly concentrated into tumors (tumor to non-tumor ratio=2.4 vs. 1.3 with (18)F-FDG). With tamoxifen treatment (n=6) (68)Ga-AMBA uptake plateaued after 1week and decreased after 2weeks, with a significant reduction compared to controls (n=4). In contrast the effect of tamoxifen treatment could not be appreciated using (18)F-FDG.CONCLUSIONS:
(68)Ga-AMBA appeared better than (18)F-FDG to visualize and monitor the response to hormone treatment in this breast cancer model.Palabras clave
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Banco de datos:
MEDLINE
Asunto principal:
Oligopéptidos
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Tamoxifeno
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Neoplasias de la Mama
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Fluorodesoxiglucosa F18
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Tomografía de Emisión de Positrones
Límite:
Animals
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Female
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Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article