Immunodetection of Na,K-ATPase alpha 3-isoform in renal and nerve tissues.
FEBS Lett
; 250(2): 545-8, 1989 Jul 03.
Article
en En
| MEDLINE
| ID: mdl-2546811
ABSTRACT
At least three types of mRNA of the catalytic subunit of Na,K-ATPase namely alpha-,alpha+- and alpha 3-isoforms are identified in different tissues. Only two of them alpha and alpha+ have well known structural and catalytic properties. Here we present immunochemical data indicating that the alpha 3 protein really exists in pig and human kidney, and human brain. Crude membrane fractions and purified membrane-bound Na,K-ATPases were immunoblotted with alpha 3-specific antibodies raised against the synthetic peptide corresponding to the unique sequence of this isoform. The mature alpha 3-subunit is shown to include the sequence GDKKDDKSSPK followed by the initiating methionine residue. Nephron collecting tubules are proposed to specifically contain Na,K-ATPase alpha 3-isoform.
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Banco de datos:
MEDLINE
Asunto principal:
ATPasa Intercambiadora de Sodio-Potasio
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Isoenzimas
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Riñón
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Microsomas
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Tejido Nervioso
Límite:
Animals
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Humans
Idioma:
En
Año:
1989
Tipo del documento:
Article