Establishment of hepatitis E virus infection-permissive and -non-permissive human hepatoma PLC/PRF/5 subclones.
Microbiol Immunol
; 59(2): 89-94, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25495578
ABSTRACT
PLC/PRF/5 cells show limited permissiveness, meaning that almost all subclones are permissive; however, some subclones do not exhibit permissiveness for hepatitis E virus (HEV) infection. In this study, the single-cell cloning of PLC/PRF/5 was performed and heterogeneous subclones characterized. Notably, the efficiency of intracellular virus replication did not correlate with the permissiveness for HEV infection. However, as well as binding permissive subclones, virus-like particles bound non-permissive subclones on various levels, suggesting that these subclones have some deficiencies in the attachment and entry steps of infection. Our data would be useful for investigating the HEV life cycle.
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Banco de datos:
MEDLINE
Asunto principal:
Replicación Viral
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Virus de la Hepatitis E
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Hepatocitos
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Acoplamiento Viral
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Internalización del Virus
Límite:
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article