Silencing of COX-2 by RNAi modulates epithelial-mesenchymal transition in breast cancer cells partially dependent on the PGE2 cascade.
Asian Pac J Cancer Prev
; 15(22): 9967-72, 2014.
Article
en En
| MEDLINE
| ID: mdl-25520137
ABSTRACT
In order to prove whether downregulation of COX-2 (Cyclooxygenase-2) could modulate the epithelial- mesenchymal transition (EMT) of breast cancer, celecoxib and siRNA were respectively used to inhibit COX-2 function and expression in MDA-MB-231 cells. The EMT reversal effect in the RNAi treated group was better than that of the celecoxib group while there were no obvious differences in the medium PGE2 levels between the two groups. The results show that COX-2 pathways may contribute considerably to EMT of breast cancer cells, partially dependent on the PGE2 cascade. Akt2, ZEB2 and Snail were measured to clarify the underlying mechanisms of COX-2 on EMT; COX-2 may modulate EMT of breast cancer by regulating these factors. This finding may be helpful to elucidate the mechanisms of selective COX-2 inhibitor action in EMT modulation in breast cancer.
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Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Dinoprostona
/
Silenciador del Gen
/
ARN Interferente Pequeño
/
Ciclooxigenasa 2
/
Inhibidores de la Ciclooxigenasa 2
/
Transición Epitelial-Mesenquimal
Límite:
Female
/
Humans
Idioma:
En
Año:
2014
Tipo del documento:
Article