Activation of autophagy via Ca(2+)-dependent AMPK/mTOR pathway in rat notochordal cells is a cellular adaptation under hyperosmotic stress.
Cell Cycle
; 14(6): 867-79, 2015.
Article
en En
| MEDLINE
| ID: mdl-25590373
Palabras clave
AMPK, AMP activated Protein Kinase; Adaptation; Apoptosis; Autophagy; BAPTA-AM, bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl) ester; DAPI, 4', 6-diamidino-2-phenylindole; ECM, extracellular matrix; EGTA, ethyleneglycol-bis (2-aminoethylether)-N,N,N', N-tetraacetic acid; FITC, fluorescein isothiocyanate; Hyperosmotic stress; IDD, Intervertebral disc degeneration; JC-1, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide; MAPK, mitogen-activated protein kinase; MDC, Monodansylcadaverine; MMP, mitochondrial membrane potential; MPT, mitochondrial permeability transition; NP, nucleus pulposus; Notochordal cells; OD, optical density; PI, propidium iodide; PLC, phospholipase C; SQSTM1, Sequestosome-1; TRPV4, Transient receptor potential vanilloid 4; fluo3-AM, fluo3 acetoximethylester; mTOR, mammalian target of rapamycin
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Presión Osmótica
/
Autofagia
/
Adaptación Fisiológica
/
Calcio
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Proteínas Quinasas Activadas por AMP
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Serina-Treonina Quinasas TOR
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Notocorda
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2015
Tipo del documento:
Article