Ryanodine and inositol triphosphate receptors modulate facilitation and tetanic depression at the frog neuromuscular junction.
Muscle Nerve
; 52(4): 623-30, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-25600698
ABSTRACT
INTRODUCTION:
Short-term plasticity of synaptic function is an important physiological control of transmitter release. Short-term plasticity can be regulated by intracellular calcium released by ryanodine and inositol triphosphate (IP3) receptors, but the role of these receptors at the neuromuscular junction is understood incompletely.METHODS:
We measured short-term plasticity of evoked endplate potential (EPP) amplitudes from frog neuromuscular junctions treated with ryanodine, 2-aminoethoxydiphenylborane (2-APB), or 1-[6-[[(17ß)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U- 73122).RESULTS:
Ryanodine decreases paired-pulse facilitation for intervals <20 ms and markedly decreases tetanic depression. Treatment with 2-APB reduces EPP amplitude, increases paired-pulse facilitation for intervals of <20 ms, and significantly reduces tetanic depression. U-73122 decreases EPP amplitude and decreases paired-pulse depression for intervals <20 ms.CONCLUSIONS:
Ryanodine, IP3 receptors, and phospholipase C modulate short-term plasticity of transmitter release at the neuromuscular junction. These results suggest possible targets for improving the safety factor of neuromuscular transmission during repetitive activity of the neuromuscular junction.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Canal Liberador de Calcio Receptor de Rianodina
/
Receptores de Inositol 1,4,5-Trifosfato
/
Unión Neuromuscular
/
Plasticidad Neuronal
Límite:
Animals
Idioma:
En
Año:
2015
Tipo del documento:
Article