In vivo assessment of mitochondrial oxygen consumption.

Harms, Floor A; Mik, Egbert G

Harms, Floor A; Mik, Egbert G.

Harms FA; Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC - University Medical Center Rotterdam, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.

Methods Mol Biol ; 1264: 219-29, 2015.

Article en En | MEDLINE | ID: mdl-25631017

ABSTRACT

ABSTRACT

The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.

Asunto(s)

Técnicas In Vitro/métodos; Mitocondrias/metabolismo; Consumo de Oxígeno; Animales; Técnicas In Vitro/instrumentación; Mediciones Luminiscentes/instrumentación; Mediciones Luminiscentes/métodos; Ratas

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Técnicas In Vitro / Mitocondrias Límite: Animals Idioma: En Año: 2015 Tipo del documento: Article

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