Stepwise changes in viable but nonculturable Vibrio cholerae cells.
Microbiol Immunol
; 59(5): 305-10, 2015 May.
Article
en En
| MEDLINE
| ID: mdl-25664673
ABSTRACT
Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Vibrio cholerae O1
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Vibrio cholerae O139
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Viabilidad Microbiana
Límite:
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article