Widespread occurrence of both metabolic and target-site herbicide resistance mechanisms in Lolium rigidum populations.
Pest Manag Sci
; 72(2): 255-63, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-25703739
ABSTRACT
BACKGROUND:
Lolium rigidum populations in Australia and globally have demonstrated rapid and widespread evolution of resistance to acetyl coenzyme A carboxylase (ACCase)-inhibiting and acetolactate synthase (ALS)-inhibiting herbicides. Thirty-three resistant L. rigidum populations, randomly collected from crop fields in a most recent resistance survey, were analysed for non-target-site diclofop metabolism and all known target-site ACCase gene resistance-endowing mutations.RESULTS:
The HPLC profile of [(14) C]-diclofop-methyl in vivo metabolism revealed that 79% of these resistant L. rigidum populations showed enhanced capacity for diclofop acid metabolism (metabolic resistance). ACCase gene sequencing identified that 91% of the populations contain plants with ACCase resistance mutation(s). Importantly, 70% of the populations exhibit both non-target-site metabolic resistance and target-site ACCase mutations.CONCLUSIONS:
This work demonstrates that metabolic herbicide resistance is commonly occurring in L. rigidum, and coevolution of both metabolic resistance and target-site resistance is an evolutionary reality. Metabolic herbicide resistance can potentially endow resistance to many herbicides and poses a threat to herbicide sustainability and thus crop production, calling for major research and management efforts.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Acetolactato Sintasa
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Acetil-CoA Carboxilasa
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Proteínas de Plantas
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Lolium
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Resistencia a los Herbicidas
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Herbicidas
País como asunto:
Oceania
Idioma:
En
Año:
2016
Tipo del documento:
Article